Seeing is Believing – Imaging the Processes of Life

J.Lippincott-Schwartz, J.Ellenberg

EMBL Heidelberg, Germany

Thursday 3 October - Sunday 6 October 2013

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Final Programme

Participants attending the EMBO|EMBL Symposium will have the opportunity to register for the optional pre-meeting Satellite Workshops taking place on Thursday 3 October 2013. An email with instructions on how to register for the workshops will be sent to all registered participants in September.

Session 1 - 11:30 – 12:30

  1. Zeiss (W1)
  2. Leica (W1)
  3. FEI Munich GmbH, formerly TILL Photonics (W1)
  4. Scientific Volume Imaging (W1)

 Session 2 - 13:00 - 14:00

  1. Zeiss (W2)
  2. Leica (W2)
  3. FEI Munich GmbH, formerly TILL Photonics (W2)
  4. Scientific Volume Imaging (W2)

For more information please visit Symposium workshops webpage.

 
Thursday, 3 October 2013
12:30-14:25 Arrival and Registration (light lunch will be available)  
14:25-14:35  Welcome / Introduction  

Session 1: Superresolution Microscopy - Sponsored by GE Healthcare
14:25-17:10

Chair: Jennifer Lippincott Schwartz

14:35-15:00 Stefan Hell MPI Goettingen, Germany Far-field optical nanoscopy: principles and recent advancements
15:00-15:25 Jan Ellenberg, EMBL Heidelberg, Germany Nuclear Pore Scaffold Structure Analyzed by Super-Resolution Microscopy and Particle Averaging
15:25-15:50 Markus Sauer, Julius-Maximilians-University Würzburg, Germany Localization microscopy is coming of age
15:50-16:15  Coffee Break
16:15-16:30 Andreas Gahlmann, Stanford University, United States of America Quantitative multicolor super-resolution imaging in three dimensions enables accurate assessments of intracellular protein co-localization
16:30-16:45 Jan Wisniewski, Janelia Farm Reserach Campus - HHMI, United States of America 3-D Multifocal Microimaging of Yeast Centromeric Histone Cse4 in Live and Fixed Cells
16:45-17:10 Rainer Heintzmann, Institute of Photonic Technology, Germany High resolution fluorescence microscopy by structured illumination
17:10-17:40 Coffee Break 
17:40-19:00 fast track session  
19:00-21:00 Dinner
20:00-21:00 Meet-the-Speakers-speed-dating-Session-Roof Top Lounge
21:00-22:30 After dinner drinks in the Roof Top Lounge - Sponsored by PCO AG

 

 


Friday, 4 October 2013
TimeTopic 

Session 2: Imaging Cytoskeleton across Scales
09:00 - 11:55

Chair: Ron Vale

09:00-09:25 Thomas Surrey, London Research Institute, United Kingdom EB1 accelerates two nanoscale transitions important for microtubule maturation and dynamics
09:25-09:50 Melina Schuh, LMB Cambridge, United Kingdom Imaging vesicle-based actin networks in mouse oocytes
09:50-10:05 Coffee Break
10:05-10:30 Ron Vale, University of California, San Francisco, USA Imaging T cell activation
10:30-10:45  Andrea Picco, EMBL Heidelberg, Germany Visualizing the dynamic architecture of the endocytic machinery by high resolution tracking of fluorescent proteins in yeast cells
10:45-11:15 Coffee Break
11:15-11:40 Jennifer Lippincott Schwartz, NICHD, NIH, USA How crawling cells create their 3D shape: new insights from superresolution imaging
11:40-11:55 Sohail Ahmed, IMB, Singapore Molecular architecture of filopodia
11:55-14:00 Lunch
13:00-14:00 Meet-the-Speakers-speed-dating-Session-ATC Foyer

Session 3: Single Molecule Imaging
14:00 - 16:05

Chair: Melissa Moore

14:00-14:25 Melissa Moore, University of Massachusetts Medical School, USA Imaging macromolecular RNA-protein machines in action
14:25-14:40 Brian English, Howard Hughes Medical Insititute, United States of America Insights into mRNA compartmentalization and translation by simultaneous single particle tracking of ribosomes and mRNAs, and by and super-resolution imaging of cytoskeletal structures in live cells
14:40-14:55 Xavier Darzacq, Ecole Normale Supérieure / CNRS, France Nuclear organization and target search processes using single molecules imaging
14:55-15:10 Coffee Break
15:10-15:35 Enrico Gratton, University of California, Irvine, USA  tbc
15:35-15:50 Katharina Gaus, University of New South Wales, Australia Molecular insights into the regulation of T cell signalling
15:50-16:05 François Waharte, Institut Curie, France  Molecular dynamics in a biological system measured by image correlation spectroscopy taking into account spatio-temporal complexity
16:05-16:30 Coffee Break

Session 4: Image Analysis
16:30 - 18:55

Chair: Gaudenz Danuser

16:30-16:55 Gaudenz Danuser, Harvard Medical School, USA Inferring causality in nonlinear cellular pathways from image fluctuations
16:55-17:20 Erik Meijering, Erasmus MC - University Medical Center Rotterdam, The Netherlands Automated Particle Tracking and Analysis: State of the Art
17:20-17:35 Nigel Burroughs, University of Warwick, United Kingdom How kinetochores move: inferring switching mechanisms from high resolution 3D tracking data
17:35-17:50 Coffee Break
17:50-18:15 Pavel Tomancak, Max Planck Institute of Molecular Cell Biology and Genetics, Germany Bioimage informatics for imaging of large biological specimen with high-resolution microscopy
18:15-18:30 Zia Khan, University of Chicago, United States of America EDGE4D a tool for quantitative 4D analyses of epithelial folding during Drosophila gastrulation
18:30-18:55 Hanchuan Peng, Allen Institute for Brain Science, USA Fast 3D visualization and human-machine interaction for high-throughput biology
18:55-20:30 Poster Session I
20:30-22:00 Dinner
21:30-22:30 Meet-the-Speakers-speed-dating-Session-Roof Top Lounge
22:30-23:30 Drinks in the ATC Roof Top Lounge - Sponsored by Zeiss

 

 

Saturday 5 October 2013
TimeTopic 

Session 5: New Biology discovered by Imaging
09:00 - 11:55

Chair: Scott Fraser

09:00-09:25 Scott Fraser, Caltech, USA Imaging molecular dynamics of embodied cells
09:25-09:40 Kota Nagasaka, The Cancer Institute of JFCR, Japan Visualization of sister chromatid resolution reveals cooperative contribution of Condensin II and Topoisomerase II alpha to mitotic chromosome assembly during prophase
09:40-09:55 Coffee Break - Sponsored by Olympus
09:55-10:20 Daniel Gerlich, IMBA - Institute of Molecular Biotechnology GmbH, Austria Imaging spindle checkpoint signaling kinetics in live cells
10:20-10:35 Jacky Goetz, INSERM, France Looking at tumor invasion and angiogenesis from a biomechanical, dynamical and correlative point of view
10:35-11:05 Coffee Break - Sponsored by Olympus
11:05-11:30 Peter Friedl, Nijmegen Centre for Molecular Life Sciences, The Netherlands Intravital infrared microscopy of tumor invasion, progression and therapy response
11:30-11:55 Roberto Weigert, National Institutes of Health/NIDCR, USA  Imaging Cell Biology in Live Animals using Intravital Microscopy
11:55-14:00 Lunch
13:00-14:00 Meet-the-Speakers-speed-dating-Session-Roof Top Lounge

Session 6: Correlative Imaging
14:00 - 16:40

Chair: Carolyn Larabell

14:00-14:25 Carolyn Larabell, University of California, San Francisco, USA Correlated fluorescence and x-ray tomography of chromosome structure
14:25-14:50 Judith Klumperman, University Medical Center Utrecht, The Netherlands Correlative light-electron microscopy of the endo-lysosomal system
14:50-15:05 Wanda Kukulski, EMBL Heidelberg, Germany Correlative microscopy resolves dynamic endocytic ultrastructures and reveals the roles of endocytic proteins in vivo
15:05-15:35 Coffee Break - Sponsored by Olympus
15:35-16:00 Pierre Gönczy, EPFL, Switzerland Imaging centriole assembly
16:00-16:15 Jean-Marc Verbavatz, Max Planck Institute of Molecular Cell Biology and Genetics, Germany 3D reconstruction of centrosomal microtubules in mitosis by electron tomography using automated segmentation
16:15-16:40 Jeff Lichtman, Harvard University, USA Connectomics
16:40-18:30 Poster Session II  
18:30 Bus shuttles depart for Heidelberg Castle 
19:00 "Gala" Dinner & Party at Heidelberg Castle

 

 

 
Sunday 6 October 2013
TimeTopic 

Session 7: Probes and Biosensors
09:00 - 12:10

Chair: Atsushi Miyawaki

09:00-09:25 Atsushi Miyawaki, RIKEN Brain Science Institute, Japan  Cruising inside X
09:25-09:40 Vsevolod Belousov, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Federation Expanding a HyPer family of genetically encoded redox probes
09:40-09:55 Coffee Break
09:55-10:20 Kai Johnsson,  EPFL, Switzerland New fluorescent probes and sensors
10:20-10:35 Yingche Chen, University of Ottawa, Canada Development of Dimaleimide Based Fluorogens and their Application for Intracellular Protein Labelling
10:35-11:05 Coffee Break
 11:05-11:20 Nela Durisic, ICFO - The Institute of Photonic Sciences, Spain Evaluation of photoactivation efficiency of fluorescent proteins at the single molecule level using an in vivo nanotemplate
11:20-11:45 Stefan Jakobs, Max Planck Institute for Biophysical Chemistry, Germany Reversibly Photoswitchable Fluorescent Proteins
11:45-12:10 Ron Germain,  National Institutes of Health, USA Spatiotemporal Analysis of the Immune System Using Both Dynamic Intravital and Highly Multiplexed Static Imaging Methods
12:10- 13:30 Lunch

Session 8: Light Sheet Microscopy
13:30 - 16:05

Chair: Eric Betzig

13:30-13:55 Eric Betzig, Janelia Farm Research Campus, USA Imaging the three-dimensional dynamics of thick fluorescent specimens at the diffraction limit and beyond
13:55-14:20 Lars Hufnagel, EMBL Heidelberg, Germany Bioimaging across scales with light-sheet microscopy
14:20-14:35 Ulrich Leischner, IPHT-Jena, Germany Generation of ultra-thin light sheets
14:35-14:50 Coffee Break
14:50-15:15 Philipp Keller, Janelia Farm Research Campus, USA Imaging development and function of the nervous system
15:15-15:30 Pantelis Tsoulfas, University of Miami School of Medicine, United States of America Light sheet fluorescent microscopy for three-dimensional analysis of traumatic CNS injuries 
 15:30-15:55 Jan Huisken, Max Planck Institute of Molecular Cell Biology, Germany The smart microscope
 15:55-16:05 Closing Remarks  
  Bus shuttle departures to downtown and Frankfurt am Main International Airport
 

 

 

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