Location & dates EMBL Heidelberg, Germany 3 - 6 Oct 2013
Deadlines Registration closed Abstract submission closed

Scientific Volume Imaging

Venue: EMBL Advanced Training Centre, Helix Room A

Workshop Sessions 1: 11:30 – 12:30

Getting started with Huygens Deconvolution; Hands-on.

Workshop Sessions 2: 13:00 – 14:00

Web-based deconvolution with Huygens Core and a Sneak Preview of Huygens Titan


We tend to believe that what we observe through the microscopic eyepiece is the real truth, but in fact microscopic images show you no more than what the microscope is willing to tell you about the object being investigated.

Artifacts like blurring, noise, and chromatic aberration all distort your image and affect the 'true' object distribution. Consequently, image interpretation and subsequent co-localization analysis and quantitative objects measurements are severely hampered.

Fortunately, the most important distortion factor of a fluorescence microscope - blurring - can be captured as the Point Spread Function (PSF). The PSF is the image of a single point object, and can be regarded as the basic building block from which your acquired image is constructed. The Huygens software uses this PSF to trace back this construction process to the original constituent object, thus restoring it. As a result, image contrast and resolution will be significantly improved, while decreasing noise. The first workshop will help you get started with deconvolving your (super-resolution) images in Huygens Essential. In the afternoon session, we will concentrate on multi-user web-based deconvolution and analysis with Huygens Core, and give a sneak preview of the upcoming Huygens TITAN.