Location & dates EMBL Heidelberg, Germany 3 - 6 Oct 2013
Deadlines Registration closed Abstract submission closed
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Zeiss

 

Venue: EMBL Advanced Training Centre, Courtyard Room A

Workshop Session 1: 11:30 – 12:30

“Superresolution microscopy in 3D” – Photoactivated Localization and Structured Illumination Microscopy to study cellular structures and dynamics

Dr. Klaus Weisshart / Carl Zeiss Microscopy GmbH

Resolving ultrafine details of subcellular structures is key to understand the organization and functioning of cellular networks.  Recent advances in far-field fluorescence microscopy provide the necessary tools to analyze these structures with resolutions well below the classical diffraction limit in all three-dimensions. These technical advances went hand in hand with improved versions of photo-switchable fluorophores that allowed to push resolution limits further down.  The wide spectrum of suitable dyes along with the high contrast achieved endow these fluorescence based superresolution (SR) techniques with the power to study the complexity of sub-cellular organelles and the relation of their constituting components down to the molecular level and under physiological conditions. They provide us in this way with a far better understanding of the assembly of macromolecular complexes and their functions within a cell than has been possible before employing conventional imaging methods. Here we will give an overview of the technical state-of-the art of two of these technologies, Structured Illumination Microscopy (SIM) and Photoactivated Localization Microscopy (PALM), and provide typical application examples in this exciting field.

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Workshop Session 2: 13:00 – 14:00

“Big Data Imaging” – How to handle the increasing amount of image data in the biological sciences.

New imaging technologies and modalities are increasing the amount of image data one can acquire rapidly. A paradigm shift towards data- and computing intensive methods in Bio-Imaging has changed the way scientists work.  Light Sheet Fluorescence Microscopy and other new trends in microscopy drive this change even further. Journals such as Science and Nature devote whole issues to this topic.

Beside the increase in hardware requirements, for evaluation and storage of data new concepts of how image data is handled and processed are needed. Smart acquisition and intelligent ways of accessing data in a world of web-based information sharing are required: Multiple users of collaborating workgroups want to readily share their data on a global basis. Furthermore, the flexibility to adapt ones workflow to individual needs is supported by open source software. Here the smooth interaction of specific software applications with the microscope commercial software packages become increasingly important. In this podium-roundtable discussion with microscopy users, facility service providers and instrument developers, we want to approach these needs and expectations with respect to future microscope and imaging systems. The goal is to improve the understanding of the respective needs and to sketch what could be done to improve the exploitation of the information provided by new optical techniques.

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