Location & dates EMBL Heidelberg, Germany 9 - 12 Oct 2019
Deadlines Registration closed Abstract submission closed

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Day 1 - Wednesday 9 October 2019
10:00-10:45 Registration for Industry Workshops and Symposium
10:45-11:45 Concurrent Industry Workshops (Registration for Symposium continues)

Registration for Symposium


Opening remarks


Session 1: Imaging across scales
Chair: Lucy Collinson, The Francis Crick Institute


Imaging cell invaders with correlative light, electron and ion microscopy
Lucy Collinson, The Francis Crick Institute, UK


Sub-Cellular Biology Throughout Intact Tissues with Axially Scanned Light-Sheet Microscopy
Kevin Dean, UT Southwestern Medical Center, USA


Nanoscale 3D neuronal imaging of milimiter sized tissues with isotropic resolution
Alexandra Pacureanu, The European Synchrotron & UCL, UK


Tailoring grids by micropatterning for in-cell cryo-electron tomography studies
Mauricio Toro-Nahuelpan, EMBL Heidelberg, Germany


3D Imaging by FIBSEM for Cell Biology with Correlation to Cryo Fluorescence Microscopy
Harald Hess, HHMI-Janelia Research Campus, USA


Coffee break


Bioimaging information
Lars Hufnagel, EMBL Heidelberg, Germany


Single-objective light-sheet microscopy with 200nm scale resolution
Reto Fiolka, UT Southwestern Medical Center, USA

15:45-16:00 Endosomal patterning of transcripts as a mechanism of local translation and signal processing
Doris Popovic, University of Zurich, Switzerland
16:00-16:15 Superresolution imaging in the kidney across scales
Hans Blom, Royal Institute of Technology, Sweden
16:15-16:45 Visualizing the Immune System: Combining Dynamic Intravital Observation with Multiplex 2D and 3D Static Tissue Imaging to Understand Host Defense and Disease
Ronald N. Germain, National Institute of Allergy and Infectious Diseases, USA
16:45-17:10 Flash talks (1 slide/2 min each): #180 Baharak Mahyad, #59 Liliana Barbieri, #175 Miguel Lopes, #282 Martin Weigert, #246 Leslie Solorzano, #287 Paul Wiseman, #281 Peiyu Wang, #91 Gautam Dey, #226 Kelly Rogers, #134 Elizabeth Haynes
17:10-19:10 Poster Session 1 (odd numbers)

Dinner in the EMBL Canteen

21:00-22:30 Welcome reception in the ATC Foyer
Day 2 - Thursday 10 October 2019

Session 2: Image analysis
Chair: Anna Kreshuk, EMBL Heidelberg


Data fusion in localisation microscopy: Combining information from many particles
Bernd Rieger, TU Delft, The Netherlands


High Resolution Imaging of Multicellular Microbial Development
using Lattice-Light Sheet Microscopy

Andreas Gahlmann, University of Virginia, USA


Probabilistic Noise2Void: Unsupervised Content-Aware Denoising
Alexander Krull, MPI-CBG/PKS Dresden, Germany


Coffee break


Automating image analysis with machine learning
Anna Kreshuk, EMBL Heidelberg, Germany


Causal Inference from Live Cell Images
Gaudenz Danuser, UT Southwestern Medical Center, USA

11:30-11:45 Whole-organism 3-D mapping of gene expression to ultrastructural tissue
and cell morphology

Christian Tischer, EMBL Heidelberg, Germany

Cellular Organelle Segmentation in Electron Microscopy
Aubrey Weigel, HHMI - Janelia, USA


Real-time 3D visualization and processing of large volumetric microscopy data
Ivo F. Sbalzarini, MPI of Molecular Cell Biology and Genetics, Germany


Lunch in the ATC Foyer &
Meet the speakers of Day 1 and Day 2 in the Courtyard Seminar Room
and Foyer (13:00 - 13:30)
Concurrent Lunchtime Workshops from Leica Microsystems
and Thermo Fisher Scientific (13:00 - 13:45)

14:30-19:00 Session 3: New biology discovered by imaging
Chair: Satyajit Mayor, National Centre for Biological Sciences

Euro-BioImaging: Europe’s Research Infrastructure for Imaging Technologies
Antje Keppler, EMBL Heidelberg, Germany


The Image Data Resource: A scalable resource for FAIR biological imaging data
Sebastien Besson, University of Dundee, UK


Centrioles in development and evolution: tiny organelles, multiple and critical functions
Mónica Bettencourt-Dias, IGC - Instituto Gulbenkian de Ciência, Portugal


A role for liquid-liquid phase separation in ESCRT-mediated nuclear envelope reformation
Alexander von Appen, University of California, San Francisco, USA


Imaging fluorescent nanoparticles reveals how cells establish nucleo-cytoplasmic compartmentalization after mitosis
Daniel W. Gerlich, IMBA - Vienna BioCenter, Austria


Expanding our understanding of neuronal transport
Lukas C. Kapitein, Utrecht University, The Netherlands

16:30-17:00 Coffee break
17:00-17:30 Imaging the skin of a living cell reveals a mechanoresponsive actin-membrane composite
Satyajit Mayor, National Centre for Biological Sciences, India
17:30-17:45 Unrevealing the molecular architecture of the centriole using a combination
of Cryo-Tomography and Ultrastructure Expansion microscopy

Paul Guichard, University of Geneva, Switzerland
17:45-18:00 Modular actin nano-architecture enables podosome protrusion and mechanosensing
Alessandra Cambi, Radboud University Medical Center, The Netherlands
18:00-18:30 MINFLUX Nanoscopy and related matters
Stefan W. Hell, MPI for Biophysical Chemistry, Germany
18:30-19:00 Eukaryotic organelles: deciphering their interdependency, structure
and dynamics with new imaging technologies

Jennifer Lippincott-Schwartz, HHMI - Janelia Research Campus, USA
19:00-19:15 Apéritif in the ATC Foyer

Dinner in the EMBL Canteen

21:00-22:30 After Dinner Drinks in the ATC Rooftop Lounge
Day 3 - Friday 11 October 2019
09:00-12:00 Session 4: New developments in super-resolution microscopy
Chair: Joerg Bewersdorf, Yale University School of Medicine
09:00-09:30 Selected Recent Advances in 3D Super-Resolution and Cryo-CLEM in Cells
W. E. Moerner, Stanford University, USA

Super-resolution structured illumination live-cell imaging with deep learning algorithm
Dong Li, Institute of Biophysics, Chinese Academy of Sciences, China


Expansion Structured Illumination Microscopy (ExSIM) as potential approach
for precise localization of mitochondrial proteins

Cristiane Benincá, MRC - MBU - University of Cambridge, UK


Coffee break


Superresolution fluorescence microscopy reveals mitochondrial organization and dynamics
Suliana Manley, École Polytechnique Fédérale de Lausanne, Switzerland


Sub-50 nm Super-resolution Microscopy for Cell Biological Research
Joerg Bewersdorf, Yale University School of Medicine, USA


Click-expansion microscopy for nanoscale imaging of non-protein biomolecules
De-en Sun, Peking University, China

11:45-12:00 Super-Resolution Across Multiple Scales by Compressive Imaging
Ola Sabet, University of Zurich, Switzerland
12:00-14:00 Lunch in the ATC Foyer
and Meet the speakers of Day 3 and Day 4 in the Courtyard Seminar Room (12:30 - 13:00)
14:00-17:00 Session 5: Probes and biosensors
Chair: Kai Johnsson, Max Planck Institute for Medical Research

A general strategy to develop cell permeable and fluorogenic probes
for multi-colour nanoscopy

Kai Johnsson, MPI for Medical Research, Germany


GenEPi: Piezo1-based fluorescent reporter for visualizing mechanical stimuli
with high spatiotemporal resolution

Periklis Pantazis, Imperial College London, UK


Manipulation of optical refractive index of mammalian cells in vivo with genetically encoded cephalopod reflectin proteins
Gerald Pao, Salk Institute, USA


Rapid cancer imaging by rationally designed fluorescence probes
Mako Kamiya, The University of Tokyo, Japan


Coffee break


Illuminating the Biochemical Activity Architecture of the Cell
Jin Zhang, University of California, San Diego, USA


Genetically encoded luminescent tools for life sciences
Atsushi Miyawaki, RIKEN Center for Brain Science, Japan


Flash talks (1 slide/2 min each): #78 David Clarke, #104 Neftali Flores-Rodriguez, #127 Lusheng Gu, #61 Andrew Barentine, #153 Friedemann Kiefer, #101 Kyong Tkhe Fam, #144 Ayako Imanishi, #109 Michelle Frei, #243 Bill Söderström, #249 Christoph Spahn


Poster Session 2 (even numbers)

19:15 Bus departures to Heidelberg Castle (only for castle dinner ticket holders)
19:30 Bus departures downtown (for free evening)
19:45-01:00 Conference Dinner and Party in Heidelberg Castle
(first come, first served - separate registration necessary)

Day 4 - Saturday 12 October 2019
09:00-12:45 Session 6: New biology super-resolved
Chair: Melike Lakadamyali, University of Pennsylvania

Super-resolution imaging of chromatin structure and dynamics
Melike Lakadamyali, University of Pennsylvania, USA


Adaptive actin networks ensure robust endocytosis
Charlotte Kaplan, University of California, Berkeley, USA


Cristae undergo continuous cycles of fusion and fission in a MICOS-dependent manner
Arun Kumar Kondadi, Heinrich Heine University, Germany


Nuclear Pores as Versatile Reference Standards for Quantitative Microscopy
Jonas Ries, EMBL Heidelberg, Germany


Coffee break


High speed single molecule dynamics in the endoplasmic reticulum
Christopher Obara, HHMI - Janelia, USA

11:15-11:30 Advanced super-resolution imaging of chromatin 'blobs'
Lothar Schermelleh, University of Oxford, UK
11:30-11:45 Multi-color single-molecule imaging uncovers extensive heterogeneity in mRNA decoding
Sanne Boersma, Hubrecht Institute, The Netherlands

Illuminating biology at the nanoscale and genome scale by imaging
Xiaowei Zhuang, Harvard University, USA


Towards resolving structural dynamics of large macromolecular machines
in cells by super-resolution microscopy

Jan Ellenberg, EMBL Heidelberg, Germany


Closing remarks and poster prize


Packed lunch


Bus departures downtown
and to Frankfurt Airport (airport shuttle tickets for sale at registration desk)